Biotechnology

World’s only 100% authentic bio-identical recombinant STGF

“Biotechnology means any technological application that uses biological systems, living organisms, or derivatives thereof, to make or modify products or processes for specific use.”

Genetic engineering is an important discipline of biotechnology and has a wide range of applications covering new drug developments, environmental conservation, energy generation and crop improvements yielding plants that could withstand various external insults. Through genetic engineering, a target (recombinant) protein could be produced efficiently using the machinery of the foster host. The engineered host may undergo fermentative cultivation to attain large quantities of the recombinant protein. Subsequent to purification to remove the contaminants, the recombinant protein is attainable as a highly pure, fully bioactive and authentic product.

Bacterial Expression Systems

1) Escherichia coli Excretion System

We have developed unique E. coli excretion systems using the Tac and LacUV5 cassettes, in conjunction with plasmid stabilizers for efficient production of recombinant proteins.

2) Bacillus subtilis Secretion System

Another expression & secretion system is engineered in B. subtilis for efficient extracellular production of heterologous proteins. Regulatory cassettes containing transcriptional controls derived from the veg I promoter and the Staphylococcal protein A signal peptide are employed in this system.

There are many advantages of the extracellular approach over the intracellular one for the production of recombinant proteins.

  •  Correct folding to a functional native form – authentic and bioactive product

  •  Reduction of proteolysis, and thus higher product yields

  •  Reduction of contamination from unwanted proteins (e.g. endotoxin), and thus attainment of product with better quality

  •  Unwanted N-terminal methionine can be avoided

  • Easy purification process and reduced production cost

  •  Suitable for large and industrial scale production

  •  Applicable for the production of all naturally secretory proteins

  •  Applicable for the production of mildly toxic proteins

  •  Continuous production possible

Patents List


  Method and Composition for treating skin wounds with epidermal growth factor

  • Wong, W.K.R., Kwong, W. Y. (2016) Improved means and method for hyper-production of authentic hbFGF in E. coli. Hong Kong Patent Application submitted.

  • Wong, W.K.R.  (2015, pending) Means and method for expression of authentic human epidermal growth factor and/or basic fibroblast growth factor in cytoplasm and/or culture medium of Escherichia coli. US Provisional Patent Application No.: 61/808.062; filed on 4 March 2013.

  • Wong, W.K.R.  (2015) Means and methods for producing authentic human basic fibroblast growth factor. US Patent No.: 9,017,968 B2; Granted on 28 April 2015.

  • Wong, W.K.R., Lam, K.H.E. and Tsang, M.W. (2003). Method and composition for treating skin wounds with epidermal growth factor. March, PCT Patent Application No.: PCT/CN03/00178.

  • Wong, W.K.R., Lam, K.H.E. and Tsang, M.W. (2003). Method and composition for treating skin wounds with epidermal growth factor. March, Taiwanese Patent Application No.: 92105257.

  • Wong, W.K.R. and Lam, K.H.E. (2000). Bacterial expression system. US Patent: 6,146,848.

  • Wong, W.K. and Chan, W.K. (2000). Cellobiase obtained from Cellulomonas biazotea. HK Patent: HK1012679.

  • Wong, W.K. and Chan, W.K. (1998). Novel cellobiase enzyme. UK Patent: GB2 289 050 B.

  • Wong, W.K. (1998). Production of human interferon α1b as an excreted recombinant protein in E. coli. PRC Patent: 1186120.

  • Wong, W.K.R. and Sutherland, M. (1990-1997). Excretion of heterologous proteins from E. coli. Worldwide Patent: Patent numbers: US 5646015 A; EP 0357 391 B1; ES 2091197T3; DE 68926895; CA 1,332,813C; US 5,223,407A; JP 2177889A; EP 357391A.

生物技術

重組鹼性成纖維細胞生長因子（bFGF）是哺乳動物和人體中一種非常微量的活性物質，對來源於中胚層和神經外胚層細胞 (如上皮細胞、真皮細胞、成纖維細胞、血管內皮細胞、骨細胞、軟骨細胞、神經元和神經膠質細胞等)具有促進修復和再生的作用。因此，bFGF具有廣泛的生理功能和重要的臨床應用價值。

1）  bFGF基因重組技術

生化提取bFGF是極其困難的，1萬個胎盤大約可提取1克bFGF。我們已成功應用基因重組技術對人bFGF基因進行修飾和重組，並獲得rh-bFGF高效表達質粒及生產型工程菌株，為實現bFGF的工業化生產奠定基礎。

2）  bFGF大規模生產工藝及品質控制技術

我們研究並掌握了rh-bFGF工程菌高密度發酵、蛋白分離純化等一系列rh-bFGF大規模生產工藝技術，並建立了完整的rh-bFGF原液品質控制體系。